Newsletter

Innovate the way we see life | Tomocube Inc. | August 2024

3D Biology Research

Recent advancements in life sciences have focused on 3D cell biology, utilizing multicellular structures like tissue sections and developing advanced 3D culture systems such as spheroids, organoids, and organ-on-a-chip.

This newsletter highlights Tomocube Holotomography as an ideal imaging technology for 3D biology. It captures the complexity of 3D structures, allows for noninvasive, live observation of tissue dynamics, and provides quantitative measurements.

[Publication] Holotomography Set New Standard for Comprehensive, Rigorous Statistical Assessments in Organoids Research

  • In this study, HT facilitates the visualization of subcellular structures of individual cells in small intestinal organoids at high spatial resolution.
  • The HT-X1 facilitates noninvasive, long term monitoring of various biological processes within the specimen.
  • Available quantitative parameters enables effective evaluation of organoid response to drug treatments.

[Video] Cancer Cells' Response to ROS - Microfluidic Imaging

  • Organ-on-a-chip, also known as microphysiological system (MPS), combines cell culture with microfluidic devices (µFDs) to better mimic the dynamic mechanical properties and biochemical functionalities of living organs.
  • In this experiment, the response of Hep3B cells to an elevated level of reactive oxygen species (ROS) were monitored and captured at 20-sec intervals over 4.5 hours.
  • A brief exposure of cancer cells to ROS was achieved by quickly passing a 2.5 mM H2O2 solution through the ibidi µ-Slide, provoking diverse phenomena including cell swelling, mitochondrial fragmentation, cytoplasmic vacuolation, and cell death.

[App Note] Visualization and Analysis of Live Organoid Dynamics with HT-X1

Download our application note to explore:

  • How HT-X1 enables subcellular-resolution visualization of intact organoids without the need for prior treatment or manipulation.
  • The visualization and quantification of hepatic organoid's growth dynamics.
  • The potential for utilizing HT's advantages organoid-based drug development and drug response assessment.