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Innovate the way we see life | Tomocube Inc. | January 2025

Regenerative Medicine

Regenerative medicine, a dynamic field at the intersection of biology, engineering, and medicine, seeks to repair or replace damaged tissues and organs by harnessing the body's natural healing processes. In this newsletter, join us to discover how holotomography is transforming regenerative science with its advanced imaging capabilities.

[Case study] Mitochondria and Bone Repair

Understanding mitochondria and aging: Holotomography captures real-time changes in mitochondrial health, a critical factor in aging and cellular energy metabolism, helping researchers explore therapeutic approaches to rejuvenate aged tissues.

  • In a recent study "Rejuvenating Aged Osteoprogenitors For Bone Repair," researchers from the University of Lausanne, Switzerland, identified mitochondrial dysfunction as a key component in age-related decline in osteoprogenitor function.
  • Holotomography imaging revealed that both intermittent fasting (IF) and nicotinamide mononucleotide (NMN) treatment preserved the 'rod-like/filament' morphology of mitochondria in oligomycin-treated cells, presenting a promising therapeutic strategy for rejuvenating bone repair.
Figure 7 (D). Representative images of mitochondrial shape alteration by oligomycin exposure ±NMN supplementation

[Case study] Mesenchymal Stem Cells and Tissue Regeneration

Stem cell therapy: By imaging stem cells in their native state, holotomography allows precise assessment of their pluripotency and potential for differentiation, laying the groundwork for various applications in regenerative science.

  • A study published in Stem Cell Research & Therapy highlights the critical role of organelle dynamics, such as the accumulation of lipid droplets (LDs) and changes in mitochondrial morphology, in determining the fate of human umbilical cord matrix-mesenchymal stem cells (hUCM-MSCs).
  • Holotomography provided detailed visualization of cellular and subcellular structures, providing insights into how fasudil and gelatin viscosity can reduce the LD accumulation, activate mitochondrial functions, and enhance hepatic differentiation of hUCM-MSCs, offering potential applications in stem cell therapy for liver regeneration.
Figure 3 (c-e). Tomographic analysis of the effect of gelatin and fasudil on hepatic differentiation. Mitochondria (red) and lipid droplets (green) were stained with MitoTracker and Biotium LipidSpot, respectively. LD quantification and volumetric analysis were performed using TomoAnalysis software by Tomocube.

[Video] 3D Cell Culture Imaging

Disease modeling and therapeutic testing: Holotomography enables detailed analysis of tissues and 3D culture systems, such as organoids, through 3D imaging, high-throughput screening, and quantitative measurement. This technology drives breakthroughs in regenerative medicine by providing a robust platform for disease modeling and therapeutic development.

Holotomography Platform for Regenerative Medicine Research

  • High resolution, label-free imaging
  • Long-term monitoring
  • 3D visualization
  • Correlative fluorescence
  • High-throughput 3D screening
  • RI-based, AI-driven quantitative analysis