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HT-2H

Revolutionary Holotomography opens a new era in label-free 3D live cell imaging

In the field of advanced imaging technology, Tomocube's very first generation of Holotomography stands out as a symbol of innovation. Leveraging groundbreaking 3D quantitative phase imaging technology, the HT-2H employs diffraction tomography to produce high-resolution 3D holographic images of unlabeled live cells and tissues. This innovative imaging series enables the rapid and straightforward acquisition of real-time, nanoscale images of individual cells, without the need for sample preparation.

By reconstructing a 3D distribution map of the refractive index of biological specimen from multiple 2D holograms, HT-2H provides researchers with essential insights into various cellular and subcellular organelle characteristics, such as volume, shape, cytoplasmic density, surface area, or deformability. Meanwhile, its long-term time-lapse imaging capability enables researchers to observe dynamic changes within samples over extended periods.

Additionally, the HT-2H seamlessly integrates the two complementary modalities of Holotomography and fluorescence imaging, excelling in its ability to offer long-term tracking of specific targets while minimizing unwanted stress to cells. Winner of the Microscopy Today 2019 Innovation Award, the HT-2H facilitates broader access for researchers and clinicians to quantitatively investigate cell pathophysiology and assess the effectiveness of drug interventions.

Features

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    Label-free 3D live cell imaging
    HT enables label-free 3D live cell imaging, providing non-invasive, real-time insights into cellular behavior.
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    Rapid image acquisition speed
    HT captures images rapidly to ensure no crucial cellular events are missed, enhancing efficiency and enabling real-time monitoring.
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    Correlative fluorescence
    HT offers precise biomolecular visualization within cells using correlative fluorescence, enhancing specificity for detailed molecular studies.
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    Providing a stable culture environment
    HT's stage top incubator enables extended timelapse studies by maintaining optimal cell culture conditions.
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    Quantitative analysis
    HT provides advanced analytical tools for accurate quantification of cellular morphology and dynamics, facilitating in-depth study of cell biology.

User benefits

  • Fast and Simple 3D Live Cell Imaging

    The Tomocube HT-2H is a powerful tool for researchers in various fields due to its label-free, high-resolution 3D imaging capabilities. By eliminating the need for sample labeling or staining, the HT-2H preserves the integrity of the sample and simplifies the imaging process, providing an excellent experience for researchers. With an optical resolution that surpasses that of conventional microscopes, the HT-2H delivers unmatched clarity, allowing researchers to visualize structures with exceptional precision. Its advanced 3D imaging function provides researchers with a comprehensive view of their samples, enabling them to explore spatial relationships and dynamic processes. Furthermore, the HT-2H has fast capture speed, capable of acquiring a 3D HT image in just 0.4 seconds (equivalent to 2.5 frames per second), facilitating rapid data acquisition and analysis. This combination of label-free imaging, high-resolution 3D imaging, and fast imaging speed empowers researchers to gain deeper insights into their samples, driving scientific exploration and breakthroughs.

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  • Streamlining Holotomography Analysis with TomoStudio

    TomoStudio, the user-friendly interface of the HT-2H, simplifies the visualization and analysis of refractive index (RI) tomograms for researchers. Its intuitive color-coding options enable easy differentiation of sample components based on their RI characteristics, facilitating comprehensive sample characterization and understanding of composition and structure. With flexible visualization features like customizable color coding and gradient transfer functions, interpreting tomographic data becomes more straightforward, offering deeper insights into sample properties. Furthermore, TomoStudio's quantitative data analysis tools effortlessly extract morphological, biochemical, and mechanical parameters, driving advancements in scientific research.

Configuration

  • HT-2H

    • Holotomographic microscopy with 3D fluorescence imaging capability
    Brochure

  • Software

    • TomoStudio for acquisition control and analysis

Accessories