Label-free 3D live cell imaging and quantification using Holotomography
Discover how holotomography offers label-free high-resolution imaging of live multicellular specimens in 3D, allowing visualization and quantification of the dynamics and mechanisms of live cells, subcellular organelles, and tissue structures without sample preparation.
In this webinar, you will discover:
· how holotomography provides label-free 4D quantitative imaging of cells, organoids, and fresh tissues;
· real-world applications of holotomography, from real-time quantification of subcellular lipid droplets to cytotoxicity assays;
· the versatility of holotomography in a wide array of research fields, including cell biology, immunology, material science, and in vitro diagnosis.
Holotomography has emerged as a useful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation. Holotomography can achieve label-free, high-resolution imaging of live specimens, including small organoids and fresh tissue samples in 3D. These samples can be observed for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, lipid droplets, etc. Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information.
This talk will present the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens. Several case studies will be introduced, such as real-time quantification of subcellular lipid droplets and liquid-liquid phase separation, bacteria classification, and cytotoxicity assay. We will also discuss how low-coherence holotomography can be a versatile tool for many research fields combining downstream molecular analysis, such as cell biology, immunology, microbiology, material science, and in vitro diagnosis.