Tomocube

1. Pipeline: (3D) FL 1Ch Segmentation & HT Quantification

This pipeline segments target objects using a single fluorescence channel and performs quantitative analysis based on holotomographic (HT) data. Fluorescence intensity is used to define object regions, and the segmented masks are then applied to the corresponding HT volume for further analysis.

It enables quantification not only of fluorescence-based features such as area and FL intensity, but also of HT-based measurements, including volume, refractive index, and dry mass.

Output: Object masks, Object instance labels, Fluorescence-based measurements, HT-based measurements
 

This pipeline segments target objects from a single fluorescence channel in 2D projections and performs quantitative analysis using holotomographic (HT) data. Fluorescence intensity is used to define object regions, and the resulting masks are applied to HT images for further quantification.

It enables measurement not only of fluorescence-based features such as area and intensity, but also HT-based parameters including refractive index, volume (projected), and dry mass.

Output: Object masks and instance labels, Fluorescence-based and HT-based measurements for each object
 

3. Pipeline: (3D) FL 1Ch Segmentation & FL Quantification

This pipeline performs segmentation and quantitative analysis using a single fluorescence channel. Target objects are segmented based on fluorescence intensity, and measurements such as object count, volume, and fluorescence intensity are extracted directly from the fluorescence data.

It provides a straightforward workflow for analyzing labeled structures in 3D fluorescence images without involving additional imaging modalities.

Output: Object masks and instance labels, Fluorescence-based measurements
 

This pipeline segments target objects using a single fluorescence channel from 2D projections and performs fluorescence-based quantitative analysis. The segmented regions are used to extract measurements such as object count, area, and fluorescence intensity.

It provides a streamlined workflow for analyzing labeled structures using fluorescence data only, without incorporating holotomographic measurements.

Output: Object masks and instance labels, Fluorescence-based measurements



Access to analysis pipelines is available upon request. Please send your inquiry to info@tomocube.com to explore the options best suited to your needs.