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Yes. TomoAnalysis Viewer provides three types of export:
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Data Export: Export TCF files to TIFF or RAW (with MHD) format. Select specific modalities, include MIP if needed, and define time frame ranges with start, end, and interval settings.
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Image Capture: Save the current view as a still image with configurable aspect ratio (Viewport, 1:1, 4:3, 16:9) and resolution upsampling up to 4×. Annotations such as scale bars and timestamps can be embedded into the captured image. In Multi View, a 2×2 composite capture is also available.
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Video Recording: The built-in Movie Maker creates videos from 3D data with configurable tracks — rotation (X/Y/Z axis), Z-slice traversal, and time-lapse playback. Multiple tracks can be chained with interpolation options including Linear, Ease In/Out, and Bezier. Frame rate (FPS) and duration are adjustable, with preview available before saving.
ROI measurement data can also be exported: ROI lists as CSV, and ROI profile graphs as PNG or JPG.
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Yes. HT captures the 3D RI structure of organoids without labeling, and TomoAnalysis Viewer lets you explore that data interactively. Switch between 2D slices, orthogonal cross-sections, and full 3D volume rendering to examine organoid morphology from any angle.
In 3D View, the Holotomography display renders the full volume, giving you a spatial overview of organoid structure. You can also enable DVR Volumes for voxel-based display within a specified RI and gradient range. Customize the display by adjusting color, transparency, and RI range, or add multiple volume layers with different color assignments. The XYZ crop range slider lets you isolate specific regions within the volume for focused inspection. -
Phase contrast and Differential Interference Contrast (DIC) microscopy are widely used techniques for visualizing unstained live cells with high contrast. However, both phase contrast and DIC microscopy offer only 2D qualitative imaging.
Holotomography (HT), on the other hand, provides quantitative 3D tomograms. This means it not only captures the structural details of the sample but also allows for precise measurements of various parameters within the sample volume. Unlike phase contrast and DIC microscopy, which rely on contrast enhancements, HT directly measures the refractive index variations within the sample, enabling detailed three-dimensional reconstructions. This quantitative aspect of HT makes it particularly valuable for studying dynamic biological processes and understanding cellular structures in greater detail. -
Refractive index (RI) is a fundamental optical property that measures how much light is bent as it enters a material. It indicates the speed of light in a given medium compared to its speed in a vacuum. This property is determined by the material's optical density and molecular structure.
RI finds applications in various fields, including bioimaging, where its label-free and quantitative properties are particularly valuable. RI-based imaging techniques offer detailed insights into biological samples' structure and composition without the need for external labels or dyes. This makes them highly useful in biomedical research and diagnostics, providing high-resolution and sensitive imaging capabilities for studying cellular morphology, composition, and dynamics.