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Download TomoAnalysis Viewer and run the installer on your Windows PC. On first launch, activate online by entering your name, organization, and email. For restricted network environments, contact info@tomocube.com for an offline activation code.
To load data, drag and drop a folder containing TCF (Tomocube Common File) data into the Explorer panel, or select File > Add Sample Data Directory to start with the included sample dataset. Double-click any dataset in the Gallery to open it in the Viewer, where you can switch between 2D View, 3D View, Orthogonal View, and Multi View modes to explore your data.
For detailed instructions, refer to the user manual — available under Help > Show Manual in the application. -
TomoAnalysis Viewer is software for viewing, evaluating, and sharing Holotomography data — before any analysis begins.
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Open instantly: Load HT datasets as-is with no format conversion. RI volumes, FL overlays, and multi-channel recordings appear with all metadata intact.
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Evaluate quickly: Inspect Z-stacks and time-lapse sequences in 2D/3D, measure line profiles and ROIs, and decide which datasets are worth full analysis.
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Compare conditions: View treatment vs. control or different time points side by side in Multi View.
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Share results: Export publication-ready figures and annotated MP4 videos directly — no external tools needed.
The Viewer is where you decide what to analyze. When you're ready to segment, quantify, or batch-process, open those datasets in TomoAnalysis.
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Phase contrast and Differential Interference Contrast (DIC) microscopy are widely used techniques for visualizing unstained live cells with high contrast. However, both phase contrast and DIC microscopy offer only 2D qualitative imaging.
Holotomography (HT), on the other hand, provides quantitative 3D tomograms. This means it not only captures the structural details of the sample but also allows for precise measurements of various parameters within the sample volume. Unlike phase contrast and DIC microscopy, which rely on contrast enhancements, HT directly measures the refractive index variations within the sample, enabling detailed three-dimensional reconstructions. This quantitative aspect of HT makes it particularly valuable for studying dynamic biological processes and understanding cellular structures in greater detail. -
Refractive index (RI) is a fundamental optical property that measures how much light is bent as it enters a material. It indicates the speed of light in a given medium compared to its speed in a vacuum. This property is determined by the material's optical density and molecular structure.
RI finds applications in various fields, including bioimaging, where its label-free and quantitative properties are particularly valuable. RI-based imaging techniques offer detailed insights into biological samples' structure and composition without the need for external labels or dyes. This makes them highly useful in biomedical research and diagnostics, providing high-resolution and sensitive imaging capabilities for studying cellular morphology, composition, and dynamics.