Phase separation research using Holotomography
Webinar on Phase separation research using holotomography (HT).
The following topics were covered:
· Brief overview of subcellular organelles and biomolecular condensates
· Conventional optical methods for the study of phase separation
· How to observe and quantify phase separation using HT
Speaker: So Young Choi (Research Assistant Professor, Dept. Chemical & Biomolecular Engineering at KAIST)
https://www.ibric.org/s.do?nFKdcwZHEDRelated publication
SHIN, Yongdae, et al. Spatiotemporal control of intracellular phase transitions using light-activated optoDroplets. Cell, 2017, 168.1: 159-171. e14. https://doi.org/10.1016/j.cell.2016.11.054
CHOI, Won Hoon, et al. Aggresomal sequestration and STUB1-mediated ubiquitylation during mammalian proteaphagy of inhibited proteasomes. Proceedings of the National Academy of Sciences, 2020, 117.32: 19190-19200. https://doi.org/10.1073/pnas.1920327117
Park, Y., Depeursinge, C. & Popescu, G. Quantitative phase imaging in biomedicine. Nature Photon 12, 578–589 (2018). https://doi.org/10.1038/s41566-018-0253-x