Label-Free Phenotypic Profiling of Platelets Using Holotomography

Platelets are challenging to study due to their small size, rapid activation, and high sensitivity to chemical labels or staining. Holotomography images live platelets in 3D without any dyes, generating a comprehensive biophysical phenotype based on refractive index that is not achievable with conventional microscopy. This note shows how HT-based phenotyping can distinguish platelet states associated with genetic mutations and infectious disease.

What's inside

- Label-free 3D imaging and biophysical quantification of live platelets (mean RI, dry mass, volume, sphericity, surface area)

- Genotype-to-phenotype translation: JAK2V617F mutation linked to elevated RI and sphericity in platelets

- Phenotypic characterization of platelets from L. donovani-infected mice (visceral leishmaniasis model)

- High-throughput large FOV acquisition (375 µm × 375 µm) with automated multi-point stitching for ~100 cells/sample

Keywords

platelet imaging label-free holotomography platelet phenotyping JAK2 mutation myeloproliferative neoplasm thrombocytopenia Leishmania blood cell imaging biophysical profiling refractive index tomography 3D cell imaging hematology

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