Label-free Observation and AI-powered Analysis of Mitochondrial Fusion and Fission Dynamics using Holotomography

Mitochondrial fusion and fission are rapid, physiologically critical events that are difficult to study long-term with fluorescence microscopy due to phototoxicity and photobleaching. Holotomography images mitochondria in live cells using their intrinsic refractive index, enabling stable, non-invasive observation of dynamic changes without labeling artifacts. Paired with the AI-powered TomoAnalysis™ pipeline, this approach delivers automated segmentation and quantitative analysis of mitochondrial morphology at the single-cell level.

What's inside

- Label-free 3D identification and real-time imaging of mitochondrial fission and fusion events at 6-second intervals

- Quantitative mitochondrial length analysis (fragmented <8 µm / elongated ≥15 µm) with drug treatment validation (Mdivi-1 and MPP+)

- AI-driven three-step pipeline in TomoAnalysis™: cell segmentation → mitochondrial segmentation → per-cell biophysical quantification

- Comparative visualization: bright field, HT, and fluorescence across short- and long-term imaging conditions

Keywords

mitochondrial dynamics mitochondrial fission mitochondrial fusion label-free imaging holotomography HT-X1 Plus TomoAnalysis AI segmentation live cell imaging refractive index tomography neurodegenerative disease metabolic disease mitochondria quantification

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