Investigating Cellular Dynamics of Live Organoids via Label-Free 3D High-Resolution Analysis with Low-Coherence Holotomography

A collaboration with IBS/POSTECH applying focus-stacked, FOV-stitched holotomography to murine small intestine organoids (sIO), resolving specialized epithelial cell types, tracking growth/maturation over 120 hours, and quantifying drug (cisplatin) response through label-free biophysical metrics.

What's inside

- Imaging/analysis workflow: focus-stacking with FOV stitching → 3D RI reconstruction → Sobel filtering → ROI segmentation → 3D quantitative analysis

- Subcellular-resolution identification of goblet, Paneth, and enteroendocrine cells within organoid epithelium, plus 16-minute time-lapse cellular dynamics

- 120-hour organoid maturation time course with depth-color-coded 3D renderings and volume/growth-rate curves

- Cisplatin vs. vehicle drug-response comparison: relative volume, protein density, and protein mass over 48 hours, showing cisplatin-induced structural breakdown

Keywords

organoid imaging small intestine organoid label-free holotomography organoid maturation drug response profiling cisplatin Paneth cell 3D organoid analysis refractive index tomography live organoid dynamics

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