Rapid, Label-free Phenotypic Profiling of Hepatic Stellate Cell Activation via Holotomography

Hepatic stellate cell activation is a key driver of liver fibrosis, but its gradual transition is difficult to monitor continuously with conventional endpoint assays that require fixation or labeling. The HT-X1™ Plus enables long-term, label-free time-lapse imaging of live HSCs, capturing the full progression of activation-associated morphological and organelle-level changes. Combined with TomoAnalysis™, this workflow delivers a quantitative biophysical fingerprint of HSC activation suitable for anti-fibrotic drug screening.

What's inside

- Label-free 3D RI imaging of HSC activation states (control, serum-starved, TGF-β1-activated) with distinct biophysical fingerprints

- Long-term time-lapse (42 h) capturing cell flattening, spread area increase, and high-RI lipid droplet depletion in real time

- Correlative HT–fluorescence validation of α-SMA and Vimentin cytoskeletal remodeling

- AI-powered TomoAnalysis™ quantification of whole-cell metrics (volume, sphericity, RI, dry mass) and organelle-level mitochondrial and lipid droplet dynamics

Keywords

hepatic stellate cell activation liver fibrosis label-free imaging holotomography HT-X1 Plus TGF-beta lipid droplet depletion time-lapse microscopy TomoAnalysis quantitative cell profiling anti-fibrotic drug screening refractive index tomography phenotypic screening

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