Characterizing Mitochondrial Fission/Fusion Dynamics via Holotomography Imaging

Demonstrates label-free, high-speed HT imaging of mitochondrial morphology in Hep3B cells, validating RI-based mitochondria identification against a fluorescent mitochondrial marker, then applying an ML-based TomoAnalysis pipeline to quantify drug-induced (Mdivi-1/MPP+) and time-dependent shifts between fragmented and elongated mitochondrial states.

What's inside

- RI line-profile validation of mitochondria against Mito-DsRed fluorescence signal

- 30-second, 6-second-interval time-lapse imaging capturing individual mitochondrial fission and fusion events

- Machine-learning (pixel classification) segmentation pipeline extracting per-mitochondrion volume, surface area, dry mass, and length

- Dose-dependent (Mdivi-1 fusion inducer / MPP+ fission inducer) and time-dependent (0–32 min) stacked bar-chart quantification of fragmented/normal/elongated mitochondria populations

Keywords

mitochondrial fission fusion holotomography label-free mitochondria imaging Hep3B cells Mdivi-1 MPP+ high-speed live cell imaging refractive index tomography TomoAnalysis mitochondrial dynamics quantification

Download the Application Note

First name*

Last name*

Title

Company*

Region*

Country*

Interested Product*

E-mail*

Comment

I have read and agree to the Terms and Policies.