Real-Time Monitoring and Advanced Visualization Strategies of the Behavior of Hepatic Organoid

Explores tailored visualization techniques — fine-sectioning, Sobel-filtered depth colorization, and gradient-based ROI masking — for revealing the temporal and spatial dynamics of murine hepatic organoids from raw HT volumes, including mitosis tracking and organoid fusion behavior.

What's inside

- Fine-focused sectional visualization capturing mitosis in an 8-minute-interval HT time-lapse

- Dimension-reduction workflow (Sobel filtering → depth colorization → maximum-intensity Z-projection) tracking two adjacent organoids over 45 minutes without fusion

- Gradient (∇RI)-based ROI extraction and masking revealing an internal 3D stem structure within a hepatic organoid

- Featured composite 3D visualization of two adjacent organoids with false-colored internal network structures

Keywords

hepatic organoid imaging holotomography visualization depth colorization Sobel filtering ROI extraction organoid mitosis label-free 3D imaging refractive index tomography organoid fusion TomoAnalysis

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